Observation of "Ruhemann's purple" is a confi | vedclass

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(C) Ninhydrin is often used to detect $\alpha$-amino acids and also free amino and carboxylic acid groups on proteins and peptides.When about $0.5 \ m

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(C) Ninhydrin is often used to detect $\alpha$-amino acids and also free amino and carboxylic acid groups on proteins and peptides.When about $0.5 \ mL$ of a $0.1\%$ solution of ninhydrin is boiled for one or two minutes with a few $mL$ of dilute amino acid or protein solution,a blue color develops.Ninhydrin degrades amino acids into aldehydes,ammonia,and $CO_2$ through a series of reactions; the net result is ninhydrin in a partially reduced form,hydrindantin.Ninhydrin then condenses with ammonia and hydrindantin to produce an intensely blue or purple pigment,sometimes called Ruhemann's purple.

Observation of "Ruhemann's purple" is a confirmatory test for the presence of

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